4.
Mermaid Woman and Barnacle Girl from Spongebob Squarepants
5.
Captain Hammer from Dr. Horrible's Sing-A-Long Blog and Captain America
6.
Magneto, and male Emma Frost and Mystique from X-Men
7.
Augustus St. Cloud from The Ventur Bros.
8.
The Doctor and her TARDIS from Doctor Who
9.
Mr. and Mrs. Captain America
11.
Tony Stark from Iron Man
13.
Luke Skywalker and Han Solo from Star Wars
14.
Sailor Man and The Sailor Scouts
16.
Bioterrorism Security Assessment Alliance from Residenti Evil
17.
A very fabulous Wonder Woman
18.
Ryu and Vega from Street Fighter
19.
The Silence from Doctor Who (It's a girl underneath the mask)
20.
More Castiels from Supernatural
22.
Cecil from Welcome To Night Vale
23.
Ed from Fullmetal Alchemist
24.
Ash(ley?) from Evil Dead
25.
Another Cecil from Welcome To Night Vale