1.Right when the first Peter Parker introduces himself as "the one and only Spider-Man," a sign in the background says "WRONG."
2.At the start of the movie, when Miles arrives to class late, the teacher is showing a video of Olivia Octavius aka Doc Ock.
3.When Miles is at his Uncle Aaron's apartment, a clip of Donald Glover wearing a Spider-Man shirt from Community Season 2, Episode 1 is playing. The episode aired shortly after the #DonaldForSpiderMan campaign back in 2010.
5.The number 42 is placed in the movie as a reference to Jackie Robinson, the first black player in MLB. Miles Morales is the first Afro-Latino superhero. You see Miles laying between the numbers four and two after he falls from a building, and the spider that originally bit him was numbered 42.
6.When Miles electrocutes Peter B. Parker, we see his nervous system lit up instead of the skeleton you typically see in animated works.
7.The background of Times Square is FILLED with details. FedEx is called RedEx, Steph Curry is a golfer instead of an NBA player (he does golf IRL), the movie Bridesmaids is called Baby Shower, and Starbucks is called Il Giornale, which was the name of coffee outlets opened by Howard Schultz before he rebranded them to Starbucks...
8.You can also see Snapchat is called Picaboo, which was its original name, Shaun of the Dead is called From Dusk Till Shaun, an ad for a Seth Rogen movie called Hold Your Horses, and Planet Hollywood is called Planet Inglewood.
9.When Miles and Peter B. Parker fall in the middle of the street, Stan Lee can be spotted stepping over them.
10.The pennant and medal hanging in Miles' room resembles the Deadpool symbol.