Scottish Conservative members who voice support for independence will be asked to hand in their membership, the party has confirmed.
Last week, Scottish Labour leader Kezia Dugdale and Scottish Liberal Democrats leader Willie Rennie both said their members would be free to campaign for independence should there be a second referendum in the future.
Labour, the Lib Dems, and the Conservatives joined forces in the last referendum to campaign as Better Together – but it now seems unlikely there would be a repeat of that campaign in the event of another referendum, with the Conservatives being the only main party which is prepared to hold a firm anti-independence party line.
Dugdale said on Sunday she wouldn't stop her party's MPs and MSPs from campaigning for independence, explaining: "If somebody holds that view on the question of independence, I'm not going to try to shut down the debate."
Scottish Conservative leader Ruth Davidson responded to Dugdale by saying it "beggars belief" that she would offer support to her parliamentarians should they opt to back independence.
Now, following Rennie's statement that he would do the same, Davidson said that leaves her party as the only one which will "stand up for the Union".
Crikey. Lib Dems now saying top brass can campaign for indy too. Clear only @ScotTories will stand up for the Union. http://t.co/5bKG0te6Lf
A spokesperson for the party confirmed to BuzzFeed News there would be "an amicable parting of ways" should Scottish Conservatives members or parliamentarians publicly back independence.
"The clue's very much in our name – it wouldn't really be in anyone's interests for a pro-independence supporter to be a member of the Scottish Conservative and Unionist party," said the spokesperson.
"We're passionately pro-UK, and anyone who's the opposite probably wouldn't want to be in the party anyway.
"In the unlikely event a politician or member decided they wanted to support the SNP, it is likely there would be an amicable parting of ways."