24. Shinzon (Star Trek: Nemesis, 2002)
23. Klaa (Star Trek V: The Final Frontier, 1989)
22. Adm. Dougherty (Star Trek: Insurrection, 1998)
21. Ayel (Star Trek, 2009)
20. The "God" of Sha Ka Ree (Star Trek V: The Final Frontier)
19. Ru'afo (Star Trek: Insurrection)
18. Sybok (Star Trek V: The Final Frontier)
17. Viceroy (Star Trek: Nemesis)
16. Maltz (Star Trek III: The Search for Spock, 1984)
15. Evil whalers (Star Trek IV: The Voyage Home, 1986)
14. Nero (Star Trek)
13. Lursa & B'Etor (Star Trek: Generations, 1994)
The only real individual villains from the Star Trek: The Next Generation TV series to carry over into the TNG movies, these buxom Klingon sisters not only collaborated with Soran (see below), but were directly responsible for the ultimate destruction of the Enterprise-D. But since they had spent so much of the TV show making Worf's life a living hell, you'd think our beloved Klingon warrior would be the one responsible for the Duras sisters' ultimate end. Nope. Their ship goes boom thanks to a technical brainstorm from Riker. Worf, and Lursa & B'Etor, deserved better. Stupid Riker.
12. Admiral Marcus (Star Trek Into Darkness, 2013)
11. The whale probe (Star Trek IV: The Voyage Home)
10. Soran (Star Trek: Generations)
9. Martia (Star Trek VI: The Undiscovered Country, 1991)
8. Kruge (Star Trek III: The Search For Spock)
7. V'ger (Star Trek: The Motion Picture, 1979)
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It may be controversial to have this so high, but hear me out: The movie itself has a deserved rep for being too ponderous and slow, but its central antagonist is quite well thought out*, and pretty terrifying. I mean, if you ignore everyone's space pajamas in the above clip, it really does still hold up as a chilling existential threat.
*SPOILER ALERT (since even serious Trek fans may have avoided seeing this snoozer): V'ger is actually Voyager 6, a simple probe sent from Earth in the 20th century and transformed into a sentient being by a race of intelligent machines. It's been collecting knowledge ever since, and now it needs to share what it's learned with its creator — and nothing will stand in the way of that mission. Very sci-fi, very Trek (and more compelling and complex than Star Trek IV's whale probe), but it would've been waaaay cooler if the movie didn't spend so much time just staring at special effects for minutes on end.
6. Ceti Alpha eels (Star Trek II: The Wrath of Khan, 1982)
5. John Harrison/Khan (Star Trek Into Darkness)
4. Lt. Valeris (Star Trek VI: The Undiscovered Country, 1991)
3. Gen. Chang (Star Trek VI: The Undiscovered Country)
2. The Borg Queen (Star Trek: First Contact, 1996)
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It was a risk placing a single leader at the center of the Borg, whose entire allure as an enemy on Star Trek: The Next Generation had been the fact that they were a relentless — and faceless — horde. But Alice Krige brought such a delicious kinkiness, and slithery intelligence, to the character, that she instantly became one of the premiere Trek baddies of all time. And what an entrance!