Prolotherapy is also known as “proliferation therapy,” “regenerative injection therapy,”or “proliferative injection therapy”. It involves injecting an otherwise non-pharmacological and non-active irritant solution into the body, generally in the region of tendons or ligaments for the purpose of strengthening weakened connective tissue and alleviating musculoskeletal pain. The precise mechanism or mechanisms of action for prolotherapy agents is currently unclear. Prolotherapy involves the injection of an irritant solution into a joint space, weakened ligament, or tendon insertion to relieve pain.Most commonly, hyperosmolar dextrose (a sugar) is the solution used;glycerine, lidocaine (a commonly used local anesthetic), phenol, and sodium morrhuate, a derivative of cod liver oil extract are other commonly used agents.The injection is administered at joints or at tendons where they connect to bone. Prolotherapy treatment sessions are generally given every two to six weeks for several months in a series ranging from 3 to 6 or more treatments.Many patients receive treatment at less frequent intervals until treatments are rarely required, if at all.